Research engineer to develop light-sheet microscopy for neurosciences applications

KEY WORDS

engineer, microscopy, neurosciences

CITY

Bordeaux

COUNTRY

France

DETAILS OF THE OFFER

Working place: Bordeaux university

Project description:

Light Sheet Fluorescence Microscopy technics (LSFM) have proven to be extremely efficient for 3D imaging of biological samples at various spatial and temporal scales with minimal photo-damaging effects. Several solutions have been developed in the field of neuroscience to image samples ranging from fixed whole brains, to single dissociated neurons growing on a coverslip. In this regard, the Interdisciplinary Institute for Neuroscience (IINS) and the Bordeaux Imaging Center (BIC) are equipped with 3 complementary LSFM techniques: (1) an ultramicroscope for whole brain imaging; (2) a Lattice Light Sheet Microscope (LLSM) to image the first layers of brain slices at high spatial resolution; (3) a home-made single objective selective plane illumination  microscope (soSPIM) dedicated to 3D cell cultures and in-depth single-molecule localization microscopy (SMLM). We aim to complete our catalog by implementing a solution based on the Oblique Plane Microscopy (OPM) architecture, which will be dedicated to fast neuronal samples imaging, ie. brain slices, equipped with local photo-manipulation and possibly other recording modalities.

Missions:

The candidate missions will be: i) to develop a custom OPM to address specific neurobiological questions, and ii) to participate to the improvement of existing light-sheet microscopes in close collaboration with developers and neuroscientists.

Candidate profile:

We seek a motivated, enthusiastic and independent candidate, with an interest in neuroscience and a strong expertise in optics and fluorescence microscopy. Complementary skills in programming and sample preparation are appreciated. The candidate will work in an English-speaking environment, in close interactions with the neuroscientists’ team of the Bordeaux Neurocampus.

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TYPE OF JOB

Tech - Admin

TYPE OF CONTRACT

CDD (temporary / 3 years)