Post-Doctoral fellow in Neurobiology and Nutrition

KEY WORDS

CITY

COUNTRY

France

DETAILS OF THE OFFER

Working place: INRAE / Bordeaux University

The French National Research Institute for Agriculture, Food, and Environment (INRAE) is a major player in research and innovation. Internationally, INRAE is among the top research organisations in the agricultural and food sciences, plant and animal sciences, as well as in ecology and environmental science. It is the world’s leading research organisation specialising in agriculture, food and the environment.

Missions:

You will be welcomed in the unit NutriNeuro UMR1286 (team NutriMind) at Bordeaux University to dissect the role of polyunsaturated fatty acids (PUFAs) on microglia maturation during brain development.

Postnatal developmental period is decisive for the optimal maturation of the mammalian brain. Programmed apoptosis is essential for proper postnatal brain development, resulting in neuronal network sculpting via elimination of in excess dead cells. Therefore, understanding brain physiological maturation cellular mechanisms is key to prevent alterations and restore an optimal neurodevelopment. Microglia, brain resident innate immune cells, have a determining role in sculpting neuronal networks via phagocytosis. The role of lipids as key players during brain development has been neglected. Long chain PUFAs largely accrete in the brain during early phases of postnatal brain development. Thus, you will aim to understand the LC-PUFAs-dependent cellular and molecular mechanisms underlying microglia maturation during brain development.

You will be in charge of:

Evaluating microglia in different in vitro models including :

• in vitro human induced pluripotent stem cell (IPSC) - derived brain cells in 2D cultures
• in vitro human 3D (cerebral organoids)
• in vivo humanized mouse models.

Using several molecular and cellular approaches will be used including gene edition technique (CRISPR-Cas9), flow cytometry (functional assays) and omics analysis.

Special conditions of activity : You will be working in collaboration with several researchers and post-docs of the team and also interacting with technicians/engineers and contributing to the everyday life of the laboratory and team.

Qualification:

Recommended training: Ph.D. in cell biology/neuroimmunology/neurobiology

Knowledge required: cell biology, in vitro cellular and molecular approaches (cell culture, transcriptomics, CRISPR-Cas9) and omics analysis (e.g. R software, RNAseq analysis) is mandatory

Appreciated experience:

• in vivo approaches using mouse models
• experience in classical molecular and cellular techniques

Skills sought: flexibility, team-player, rigor, motivation, enthusiasm, organizational skills

+info

TYPE OF JOB

TYPE OF CONTRACT

CDD (temporary / 1 year renewable)

REMUNERATION

APPLICATION DEADLINE

EMPLOYMENT START DATE