DETAILS OF THE OFFER | Working place: Institut Cochin - Functional Pharmacology and Pathophysiology of Membrane Receptors Missions: - The PhD student will use techniques of molecular biology to design and develop new biosensors, and cellular biology to express the sensors in a cellular context (neuronal and non-neuronal) and develop stable biosensor cell lines.
- Biosensor cells will be challenged with different stimuli to identify conditions that favor or impair the pathological conformational change or aggregation of tau. Biosensors will also be used to validate drug candidates suspected to interfere with tau aggregation/seeding.
- The student will also participate in the in vivo experiments of monitoring of tau aggregation by in vivo bioluminescence imaging.
- The main techniques to be performed by the student comprise: cell culture and cell signaling, molecular cloning, nanoluciferase complementation assay, western blot, confocal microscopy, in vivo bioluminescence, immuno-histology/cytology.
Qualifications:
- Excellent communication skills in English, orally and written.
- The team members are from a diversity of nationalities, thus, English is the most used
language - Good organization and scientific rigor skills
- Curiosity and Motivation for scientific projects
- Ability to solve problems
- Team-oriented personality
Specific Requirements: • Experience in mammalian cell culture
• Experience in molecular biology and cell biology
• Experience in analysis of protein expression by western-blot and by
immunofluorescence analysis of cells in confocal microscopy
• Experience in animal experimentation
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